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LAB RESEARCH ANALYST I - Human Vaccine Institute

at Duke University Hospital

Posted: 9/25/2019
Job Reference #: 1365210
Keywords:

Job Description

Auto req ID
112762BR

Duke Entity
MEDICAL CENTER

Job Code
1888 LAB RESEARCH ANALYST I

Job Description
Laboratory Research Analyst I
 
A HIV-1 Envelope (Env) vaccine that elicits broadly neutralizing antibodies (bnAbs) has remained elusive, in part due to our limited understanding of the mechanisms of bnAb induction during natural infection. Recently developed simian-human chimeric immunodeficiency viruses (SHIVs) bearing Envs from HIV-1 transmitted-founder (TF) viruses associated with bnAb induction in HIV-1 infected adult individuals now provide an experimental model to establish the mechanisms of bnAb development. HIV-1 Env bnAbs develop in ~50% of chronically-infected adults in over 3-5 years of infection, but HIV-1-infected infants and children more frequently develop bnAbs and do so faster than adults. Understanding the cellular and molecular mechanisms of infant bnAb development will facilitate design of vaccine strategies to elicit bnAbs. This research is to define neonatal bnAb immunity to novel SHIVs that can be recapitulated with a vaccine. The Duke Human Vaccine Institute provides an excellent environment for academic and professional growth while employing our core values of collaboration, innovation and excellence.
 

Responsibilities
 
Perform isolation and characterization of HIV-1 Envelope-reactive B cells via FACS
 
Perform isolation of antibody genes using molecular techniques, including PCR and next generation deep sequencing for characterization of B cell responses to virus infection and vaccination
 
Perform characterization of T cell responses using peptide-based ELISpot
 
Perform transcriptomics of immune cells
 
Perform computational analysis of large immunoglobulin gene sequence data sets

Design, execute, analyze, and archive experiments in a manner that is scientifically justifiable, reliable and understandable to others.

Track work and compliantly order materials and reagents associated with numerous projects under multiple grants.

Follow all standard operating procedures when conducting research and review all experimental results with primary investigator/supervisor.

Education, Skills, & Experience Preferences
  • Bachelor’s degree in biological science or related field and two years research experience required.  Masters in biological science or related field is preferred.
  • Molecular biology research experience
  • Must be able to manage multiple and rapidly changing priorities and have ability to quickly learn new skills
  • Must be detail-oriented, well organized with strong communication skills and ability to work in an interactive team environment.


Location
Durham

Requisition Number
401588589

Position Title
LAB RESEARCH ANALYST I - Human Vaccine Institute

Shift
First/Day

Job Family Level
11

Full Time / Part Time
FULL TIME

Regular / Temporary
Regular

Department Name
Duke Human Vaccine Institute - Admin

Minimum Qualifications
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Education

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE